Protein Purification Reagents and Kits
Mayflower Bioscience Exo-spin 20 mini-HD columns
Size exclusion chromatography (SEC) columns are a reliable method for exosome isolation and can be used as a stand-alone product or as part of a combined strategy for isolating small EVs. The EX05 Exo-spin™ kit contains packed and equilibrated ready-to-use SEC columns.
Invent Biotechnologies Inc Saliva Exosome Isolat Kit
Diagnoses using exosomes derived from saliva have been attracting great attention in recent years due to the ease of sample collection. However, saliva samples are challenging when it comes down to exosome isolation. In addition to cells and cell debris, large amount of amylase, mucin and glycoprotein are present in saliva, and make the samples viscous and hard to manipulate. Pre-treatments, such as sonication and dilution, are often required. In many cases, the saliva samples can still be difficult to handle even after pre-treatments. This product is specifically designed to address these issues using the proprietary saliva filters. Highly viscous saliva samples can be converted to non-viscous solutions instantly by passing the samples through the filter. Exosomes can then be readily precipitated from as little as 100 µl saliva using the highly effective non-PEG-based reagent.
Mayflower Bioscience Exo-spin, 8 columns, 60 ml buffer
The EX03 Exo-spin™ kit only contains pre-packed and equilibrated ready-to-use SEC columns as the precipitation step might not be recommended for some applications (e.g. mass spectrometry) or not needed for small volumes of sera sample. The EX03 Exo-spin™ kit has been developed to process any sample smaller than 100 µl. Plasma contains much higher protein levels than sera and can only be processed directly using the EX02 or EX04 Exo-spin™ kit. EX03 Exo-spin™ kit may be used to clean-up samples which have been purified by other methods, such as precipitation and ultracentrifugation, in order to remove proteins and smaller molecules. Exo-spin™ columns are also very effective for the removal of excess dye in fluorescently labelled exosome samples.
Invent Biotechnologies Inc Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes - Spin Colum-Based (20 Preps)
Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes are essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into nucleus and cytosolic fraction is a common practice in the lab. However, when it comes to adipocytes, separation of these two fractions is much more difficult because of the high concentration of lipid droplet and low protein content of adipocytes. Methods reported in the literature are tedious and time consuming and as much as 50 grams of tissue are required. This kit provides a very rapid and simple method for obtaining high purity nuclei from adipose tissues and most important of all, only milligram amounts of tissues are required making it possible to isolate nuclei and cytosol from small animal and biopsy samples.
Invent Biotechnologies Inc Minute™ ER Enrichment Kit - spin column-based (20 Preps)
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. ER plays a central role in exocytic pathway of protein trafficking in all eukaryotic cells. Proteins synthesized in cytoplasm are translocated into ER from where vesicles shuttle protein cargo to the Golgi apparatus, which subsequently fuses with plasma membrane. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming, and contain significant cross-contamination. . Unlike any other ER isolation kit on the market, our ER isolation kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of starting cultured cells or tissues. This kit can differentially precipitate native ER from cultured cells/tissues without using a Dounce homogenizer or ultracentrifugation.
Mayflower Bioscience Exo-spin standard, 24 columns, 250 ml buffer
The EX01 Exo-spin™ kit has been developed to process up to 50 ml of cell culture medium, saliva, urine, cerebrospinal fluid (CSF), human breast milk and other low-protein biological fluids. The kit includes Exo-spin™ buffer as well as SEC columns. The Exo-spin™ buffer is a polymer-based precipitation buffer and will be used for the first step. For the second step, pre-packed and equilibrated ready-to-use SEC columns are included.
Bioworld GST-Tagged Protein Purification Buffer Kit, 200 mL
bio-World's GST-Tagged Protein Purification Buffer Kit consists of a set of buffers necessary for OnePass™ GST-Tagged Recombinant Protein Purification System(Glutathione-Separopore conveniently prepacked spin columns for gravity flow chromatography) or batch purification using Glutathione Separopore.Kit Contents:• GST Binding Buffer (100 ml)• Wash Buffer (200ml)• GST Elution Buffer (50ml)
Gyros Inc Gyrolab Bioaffy 1000 HC Assay Toolbox, 1000 nL sample volume, high binding capacity, 96 data points per CD
Gyrolab Bioaffy 1000 HC Assay Toolbox, 1000 nL sample volume, high binding capacity, 96 data points per CD
Hampton Research HR2-931-05/StockOptions Sodium HEPES #05 pH 7.2, 185 ml
HR2-931-05/StockOptions Sodium HEPES #05 pH 7.2, 185 ml. Please note items from this supplier are non-returnable.
Cytek Biosciences Inc cFluor® V450 Anti-Human CD45RA (HI100) 100test
The HI100 monoclonal antibody binds to the 220 kDa isoform of the human leukocyte common antigen. CD45RA is an isoform of CD45 due to alternate splicing1. CD45RA is expressed on naïve or resting CD4+ and CD8+ T cells, as well as on a subset of activated T cells2,3. The associated tyrosine phosphatase activity regulates signal transduction processes during hematopoiesis and immune responses4.The antibody was conjugated to a fluorophore and purified by affinity chromatography.
Biotage IMAC PHYTIP COLUMNS 1ML
IMAC PhyTip columns (1 mL volume) containing 20 ul affinity media per column, Tecan. Incl. buffer kit
Miltenyi Biotec, Inc. Adult Neuron Iso Kit, mouse - Small Size
The Adult Neuron Isolation Kit, mouse depletes all non-neuronal cells to acquire highly purified neurons from mouse brain tissue older than postnatal day 7, resulting in superior performance in single cell sequencing and cell culture.